MIF-2 significantly reduces cardiac infarction in rodents undergoing cardiac ischemia-reperfusion injury.
Also known as D-dopachrome tautomerase (D-DT), MIF-2 is a mammalian protein that is expressed by many cell types. Under stress, such as ischemia, cells secrete this protein to induce autocrine-paracrine signaling in the ischemic tissue. MIF-2 is related to MIF protein but lacks the protein domain mediating inflammatory effects and instead exclusively binds CD74. By binding to this receptor, MIF-2 initiates a signaling cascade through AMP kinase and others mediators to alter the cells metabolically and transcriptionally, thereby enabling the cells to survive the ischemia-reperfusion injury.
MIF-2 is a 37.5 kD homotrimeric protein with no post-translational modifications, and can be efficiently and inexpensively produced in E. coli.